Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Testobolin is intended to treat low testosterone levels . Taking it when levels are adequate can contribute to more surprising results than expected.
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Use of 17 alpha-alkylated anabolic-androgenic steroids (17alpha-AAS) has been connected to hepatotoxicity. These steroids are used clinically to treat anemia, to prevent weight loss, and to treat wasting syndrome. The most common types of 17alpha-AAS are Methyltestosterone, Oxandrolone, Oxymetholone and Stanozolol. Liver disease and the effects of some anti-HIV drugs may contribute to hepatic dysfunction. Signs of hepatic dysfunction are listed. For those experiencing jaundice and related malfunctions, discontinuing the drug enables patients to recover. In many cases those who did not exhibit jaundice may have developed a tolerance for the drugs. Side effects such as cholestatic jaundice only occurred in a small number of patients taking the recommended doses of 17alpha-AAS. Peliosis hepatitis, hepatic tumors, and hepatocellular adenomas are other reported side effects. Proper dosing and monitoring of anabolic steroids reduces the risk of hepatotoxicity.